As a postdoc researcher at the Institute of Analytical Chemistry of the Czech Academy of Sciences, I was introduced to the electromigration separation techniques. I started with capillary zone electrophoresis with laser-induced fluorescence detection of biologically important molecules such as nucleic acids, proteins, peptides, and antibodies, which were labeled with luminescent quantum dots. Also, the method was applied for the determination of the number of charges and organic molecules bonded to the nanocrystal surface (Electrophoresis, 36, 867-87 (2015)). Also, the influence of the prepared nanoparticles on chemiluminescence was studied (Journal of Luminescence, 184, 235-241 (2017)). Consequently, I was interested in isoelectric focusing, another electromigration method. I worked on the development of carrier ampholytes mixture compatible with mass spectrometry for protein separation. Since 2016, I have been interested in the isotachophoretic method and its application in the purification of biologically important molecules, mainly nucleic acids (Journal of Separation Science, 41, 236–247 (2018)). I started with optimization of conditions for nucleic acid concentration and purification on a commercial instrument with a focus on a relatively large volume of injected sample, recovery, purity, and large scale of nucleic acids fragments (Journal of Chromatography A, 1548, 100-103 (2018)). Since the amount of biologically relevant compounds is relatively small compared to the sample volume of the matrix, we decided to design a device which can be able to deal with a large sample volume. The enhanced device allows us to concentrate and purify analyte from up to several millilitres (Analytical Chemistry,91, 11, 7047-7053 (2019)).
Poster presentation: Epitachophoresis – media stabilizing LE and TE border